PICALM Antibody (PSH01-64)

Western Blot: PICALM Antibody (PSH01-64) [NBP3-32822]

Western Blot: PICALM Antibody (PSH01-64) [NBP3-32822] - Western blot analysis of PICALM on different lysates with Rabbit anti-PICALM antibody (NBP3-32822) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: PC-3M cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: 293T cell lysate
Lane 6: Neuro-2a cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 71 kDa
Observed band size: 65/71 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32822) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.