Piccolo Antibody (PCLO-01)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62209

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow (Intracellular), Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCLO-01

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Piccolo Antibodies »

Product Specifications

Immunogen

Human recombinant PCLO protein

Specificity

The antibody A53-B/A2 reacts with Rod domain of cytokeratin 19 (40 kDa) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

400 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Piccolo Antibody (PCLO-01)

Western Blot: Piccolo Antibody (PCLO-01) [NBP2-62209]

Western Blot: Piccolo Antibody (PCLO-01) [NBP2-62209]

Western Blot: Piccolo Antibody (PCLO-01) [NBP2-62209] - Human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb PCLO-01 and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting.
Piccolo Antibody (PCLO-01) Western Blot: Piccolo Antibody (PCLO-01) [NBP2-62209]

Western Blot: Piccolo Antibody (PCLO-01) [NBP2-62209]

Staining of human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb PCLO-01 and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting.

Applications for Piccolo Antibody (PCLO-01)

Application
Recommended Usage

Flow (Intracellular)

1-5 ug/ml

Flow Cytometry

1-5 ug/ml

Western Blot

1:100-1:2000
Application Notes
A small splice variant of PCLO can be analyzed by Western blotting, however, the whole protein is too large (over 400 kDa) to be successfully processed by SDS PAGE.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Piccolo

Piccolo is a presynaptic cytomatrix protein concentrated at the presynaptic side of synaptic junctions. Piccolo is a large protein which constists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. It is usually found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1. Piccolo is highly expressed in brain with low levels found in stomach. It is not detected in other tissues analyzed including adrenal gland, testis and pancreas.

Long Name

Presynaptic Cytomatrix Protein

Alternate Names

ACZ, Aczonin, PCLO

Gene Symbol

PCLO

Additional Piccolo Products

Product Documents for Piccolo Antibody (PCLO-01)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Piccolo Antibody (PCLO-01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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