PIEZO1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78446

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Guinea Pig

Cited:

Human, Mouse, Canine, Guinea Pig

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PIEZO1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human PIEZO1 protein (between residues 950-1000) [UniProt Q92508]

Reactivity Notes

Guinea Pig reactivity reported in scientific literature (PMID: 30324494).

Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane; Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIEZO1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody - BSA Free [NBP1-78446]

Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody - BSA Free [NBP1-78446]

Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78446] - PIEZO1 antibody (Goat anti-rabbit 568) was tested in MDCK cells. Nuclei and B-actin were counterstained with DAPI (blue) and phalloidin (green). Image from verified customer review.
Immunohistochemistry: PIEZO1 Antibody - BSA Free [NBP1-78446]

Immunohistochemistry: PIEZO1 Antibody - BSA Free [NBP1-78446]

Immunohistochemistry: PIEZO1 Antibody [NBP1-78446] - Analysis of PIEZ01 in mouse epidermis using DAB with hematoxylin counterstain.
Flow Cytometry: PIEZO1 Antibody - BSA Free [NBP1-78446]

Flow Cytometry: PIEZO1 Antibody - BSA Free [NBP1-78446]

Flow Cytometry: PIEZO1 Antibody [NBP1-78446] - An intracellular stain was performed on MCF7 cells with PIEZO1 Antibody NBP1-78446 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
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Applications for PIEZO1 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1-5 ug/ml. Use reported in scientific literature (PMID 31685811)

Immunocytochemistry/ Immunofluorescence

1:25

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:400

Western Blot

2 ug/ml
Application Notes
According to Gudipaty et al "only this Piezo1 antibody reliably worked for Piezo1 immunostaining, as it vanishes in cells mosaically expressing a Piezo1-shRNA-mCherry construct" (PMID: 28199303). Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 1 review rated 3 using NBP1-78446 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIEZO1

Piezo1 (Mib, FAM38A) is a mechanosensitive ion channel which responds to mechanically activated currents in numerous cells, including the brain, bladder, colon, kidney, lung, and skin. However, mRNA expression of Piezo1 is weak in the DRG, unlike Piezo2. The expression of Piezo1 is induced in Alzheimer's associated senile plaques (PMID 16854388), as well as integrin activation through R-Ras signaling (PMID 20016066). R-Ras is recruited to the endoplasmic reticulum which increases calcium release, leading to the activation of the protease calpain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins.

Long Name

Piezo-type mechanosensitive ion channel component 1

Alternate Names

FAM38A, KIAA0233, Mib, Protein FAM38A

Entrez Gene IDs

9780 (Human)

Gene Symbol

PIEZO1

UniProt

Q92508 (Human)

Additional PIEZO1 Products

Product Documents for PIEZO1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIEZO1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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