PIEZO2 Antibody - BSA Free

Immunohistochemical Analysis of PIEZO2 in Mouse Epidermis

Analysis of PIEZ02 in mouse epidermis using DAB with hematoxylin counterstain.

Immunohistochemical Staining of PIEZO2 in Paraffin Embedded Human Breast Tissue

Analysis of a FFPE tissue section of human breast using 1:200 dilution of PIEZO2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Immunocytochemistry/ Immunofluorescence: PIEZO2 Antibody - BSA Free [NBP1-78624] -

Immunocytochemistry/ Immunofluorescence: PIEZO2 Antibody - BSA Free [NBP1-78624] - Suppression of MA in MTN neurons from adult C57BL/6J mice by silencing Piezo2.(a) Schematic representation of the unilateral adeno-associated viral (AAV) injection site targeting MTN neurons. (b) Fluorescence images showing the expression of GFP-tagged MTN neurons in brain slices following AAV delivery. (b’) Higher magnification of the boxed area in (b). (c) The AAV-Piezo2-sh-1 construct. (d) Brainstem coronal sections of C57BL/6J mice infected with the AVV-shRNA against Piezo2 (middle) & the scrambled AVV (lower), showing MTN neurons immunolabeled for Piezo2 (left, red) or GFP (middle, green), & the merged image of both, with the nuclei stained with DAPI (blue). Arrowheads indicate representative MTN neurons. Scale bar 50 μm. (e) Traces of MA currents evoked by mechanical indentation in MTN neurons infected with AAV-Piezo2-sh-1 or with a scrambled AVV (f). (g) Trace from a MTN neuron infected with AAV-Piezo2-sh-1 that responded with RA mechanical activated currents ( tau = 6.2 ms). The inset shows a higher magnification of the current trace corresponding to a 7 μm displacement. The black upper trace shows the 250 ms mechanical stimulus steps in 1 μm increments applied every 10 s, the breaks in the trace corresponding to equivalent breaks in the recording. (h) Histogram summarizing the mean amplitude of the RA currents in MTN neurons from WT mice (n = 69) & those infected with AAV-Piezo2-sh-1 (n = 20) or AAV-shScr (n = 8). The data are expressed as the means ± s.e.m.: *p < 0.05 (Student’s t-test). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep25923), licensed under a CC-BY license. Not internally tested by Novus Biologicals.