PIGN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81248

Novus Biologicals, part of Bio-Techne
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NBP1-81248
NBP1-81248-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PIGN Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KVDDGVKEIVSMFNHFYGNDGKTTFIFTSDHGMTDWGSHGAGHPSETLTPLVTWGAGIKYPQRVSAQQFDDAFLKEWRLENWK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIGN Antibody

Immunocytochemistry/ Immunofluorescence: PIGN Antibody [NBP1-81248]

Immunocytochemistry/ Immunofluorescence: PIGN Antibody [NBP1-81248]

Immunocytochemistry/Immunofluorescence: PIGN Antibody [NBP1-81248] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunocytochemistry/ Immunofluorescence: PIGN Antibody [NBP1-81248]

Immunocytochemistry/ Immunofluorescence: PIGN Antibody [NBP1-81248]

Immunocytochemistry/Immunofluorescence: PIGN Antibody [NBP1-81248] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.

Applications for PIGN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIGN

PIGN encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is expressed in the endoplasmic reticulum and transfers phosphoethanolamine (EtNP) to the first mannose of the GPI anchor. Two alternatively spliced variants, which encode an identical isoform, have been reported. Transcript Variant: This variant (1) represents the longer variant. Variants 1 and 2 encode the same isoform.

Alternate Names

EC 2.-, GPI ethanolamine phosphate transferase 1, MCD4, MCD4 homolog, MDC4, MGC26427, phosphatidylinositol glycan anchor biosynthesis, class N, Phosphatidylinositol-glycan biosynthesis class N protein, PIG-Nphosphatidylinositol glycan, class N

Gene Symbol

PIGN

Additional PIGN Products

Product Documents for PIGN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PIGN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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