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PIGP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84294

Novus Biologicals, part of Bio-Techne
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NBP1-84294-25ul
NBP1-84294

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NMMSTSPLDSIHTITDNYAKNQQQKKYQEEAIPALRDISISEVNQMFFLAAKELYTK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIGP Antibody

Western Blot: PIGP Antibody [NBP1-84294]

Western Blot: PIGP Antibody [NBP1-84294]

Western Blot: PIGP Antibody [NBP1-84294] - Analysis in control (vector only transfected HEK293T lysate) and PIGP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: PIGP Antibody [NBP1-84294]

Immunocytochemistry/ Immunofluorescence: PIGP Antibody [NBP1-84294]

Immunocytochemistry/Immunofluorescence: PIGP Antibody [NBP1-84294] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Immunohistochemistry-Paraffin: PIGP Antibody [NBP1-84294]

Immunohistochemistry-Paraffin: PIGP Antibody [NBP1-84294]

Immunohistochemistry-Paraffin: PIGP Antibody [NBP1-84294] - Staining of human stomach shows strong membranous positivity in parietal cells.

Applications for PIGP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIGP

The PIGP gene encodes an enzyme involved in the first step of glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. The encoded protein is a compone

Alternate Names

class P, DCRCDown syndrome critical region protein C, Down syndrome critical region protein 5, DSCR5DCRC-S, DSCRC, DSRC, EC 2.4.1.198, phosphatidylinositol glycan anchor biosynthesis, class P, Phosphatidylinositol-glycan biosynthesis class P protein, phosphatidylinositol-n-acetylglucosaminyltranferase subunit, PIG-P

Gene Symbol

PIGP

Additional PIGP Products

Product Documents for PIGP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIGP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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