PIK3CA Antibody (3G3)
Novus Biologicals, part of Bio-Techne | Catalog # H00005290-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Proximity Ligation Assay, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3G3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PIK3CA (NP_006209, 959 a.a. ~ 1068 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQHALN
Specificity
PIK3CA (3G3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for PIK3CA Antibody (3G3)
![Proximity Ligation Assay: PIK3CA Antibody (3G3) [H00005290-M01]](https://resources.bio-techne.com/images/products/PIK3CA-Antibody-3G3-Proximity-Ligation-Assay-H00005290-M01-img0002.jpg "Proximity Ligation Assay: PIK3CA Antibody (3G3) [H00005290-M01]")
Proximity Ligation Assay: PIK3CA Antibody (3G3) [H00005290-M01]
Proximity Ligation Assay: PIK3CA Antibody (3G3) [H00005290-M01] - Analysis of protein-protein interactions between APPL1 and PIK3CA. HeLa cells were stained with anti-APPL1 rabbit purified polyclonal 1:1200 and anti-PIK3CA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: PIK3CA Antibody (3G3) [H00005290-M01] - Detection limit for recombinant GST tagged PIK3CA is approximately 1ng/ml as a capture antibody.
Applications for PIK3CA Antibody (3G3)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Proximity Ligation Assay
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PIK3CA
Long Name
PI3-Kinase P110 Subunit Alpha
Alternate Names
CLAPO, CLOVE, CWS5, MCMTC, P110alpha, PI3Kalpha
Entrez Gene IDs
5290 (Human)
Gene Symbol
PIK3CA
OMIM
114480 (Human)
UniProt
Additional PIK3CA Products
Product Documents for PIK3CA Antibody (3G3)
Product Specific Notices for PIK3CA Antibody (3G3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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