PIK3R5 Antibody (OTI4G9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46413

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4G9

Concentration

1 mg/ml

View all PIK3R5 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PIK3R5(NP_055123) produced in HEk293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

97.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIK3R5 Antibody (OTI4G9)

Western Blot: PIK3R5 Antibody (OTI4G9) [NBP2-46413]

Western Blot: PIK3R5 Antibody (OTI4G9) [NBP2-46413]

Western Blot: PIK3R5 Antibody (OTI4G9) [NBP2-46413] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3R5.
Immunocytochemistry/ Immunofluorescence: PIK3R5 Antibody (OTI4G9) [NBP2-46413]

Immunocytochemistry/ Immunofluorescence: PIK3R5 Antibody (OTI4G9) [NBP2-46413]

Immunocytochemistry/Immunofluorescence: PIK3R5 Antibody (OTI4G9) [NBP2-46413] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PIK3R5.
Immunohistochemistry: PIK3R5 Antibody (OTI4G9) [NBP2-46413]

Immunohistochemistry: PIK3R5 Antibody (OTI4G9) [NBP2-46413]

Immunohistochemistry: PIK3R5 Antibody (OTI4G9) [NBP2-46413] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for PIK3R5 Antibody (OTI4G9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIK3R5

Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of 85 kDa (p85) and 110 kDa (p110) subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85alpha and p85beta), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110alpha and p110beta interact with p85alpha, and p110alpha has also been shown to interact with p85beta in vitro. p110delta expression is restricted to white blood cells. It has been shown to bind p85alpha and beta, but it apparently does not phosphorylate these subunits. p110delta seems to have the capacity to autophosphorylate. p110gamma does not interact with the p85 subunits. It has been shown to be activated by alpha and betagamma heterotrimeric G proteins.

Long Name

Phosphoinositide-3-kinase, Regulatory Subunit 5

Alternate Names

FOAP-2, p101-PI3K

Gene Symbol

PIK3R5

Additional PIK3R5 Products

Product Documents for PIK3R5 Antibody (OTI4G9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIK3R5 Antibody (OTI4G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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