PIN/DLC8 Antibody (1H7)

Novus Biologicals, part of Bio-Techne | Catalog # H00008655-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1H7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PIN Antibodies »

Product Specifications

Immunogen

DYNLL1 (NP_003737.1, 1 a.a. ~ 72 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MCDRKAVIKNADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKH

Specificity

DYNLL1 - dynein, light chain, LC8-type 1 (1H7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PIN/DLC8 Antibody (1H7)

Western Blot: PIN/DLC8 Antibody (1H7) [H00008655-M02]

Western Blot: PIN/DLC8 Antibody (1H7) [H00008655-M02]

Western Blot: PIN/DLC8 Antibody (1H7) [H00008655-M02] - Analysis of DYNLL1 expression in transfected 293T cell line by DYNLL1 monoclonal antibody (M02), clone 1H7.Lane 1: DYNLL1 transfected lysate(10.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PIN/DLC8 Antibody (1H7) [H00008655-M02]

Immunocytochemistry/ Immunofluorescence: PIN/DLC8 Antibody (1H7) [H00008655-M02]

Immunocytochemistry/Immunofluorescence: PIN/DLC8 Antibody (1H7) [H00008655-M02] - Analysis of monoclonal antibody to DYNLL1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: PIN/DLC8 Antibody (1H7) [H00008655-M02] - Detection limit for recombinant GST tagged DYNLL1 is 0.1 ng/ml as a capture antibody.

Applications for PIN/DLC8 Antibody (1H7)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PIN

Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized. [provided by RefSeq]

Long Name

Protein Inhibitor of Neuronal Nitric Oxide Synthase

Alternate Names

DLC1, DLC8, DNCL1, DNCLC1, DYNLL1, LC8a

Entrez Gene IDs

8655 (Human)

Gene Symbol

DYNLL1

UniProt

NP_003737 (Human)

Additional PIN Products

Product Documents for PIN/DLC8 Antibody (1H7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIN/DLC8 Antibody (1H7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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