Pin1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-53805

Novus Biologicals, part of Bio-Techne
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NB100-53805
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Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Canine (100%), Human (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Pin1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KHSQSRRPSSWRQEKITR corresponding to internal region according to NP_006212.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Pin1 Antibody

Western Blot: Pin1 Antibody [NB100-53805]

Western Blot: Pin1 Antibody [NB100-53805]

Western Blot: Pin1 Antibody [NB100-53805] - (0.2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Pin1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.2 - 0.6 ug/ml
Application Notes
WB: Approx. 17 kDa band observed in rat brain lysates (calculated MW of 18.2 kDa band according to human NP_006212.1 and 18.2 kDa band according to mouse NP_075860).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pin1

Pin1 is a Peptidyl-prolyl isomerases (PPIase). Peptidyl-prolyl isomerases (PPIase) facilitate the cis-trans interconversion of the peptidyl-prolyl bond thereby affecting protein folding. Pin1 is a PPIase which specifically recognizes phosphorylated S/T-P bonds. Pin1 has been implicated in tau pathologies that underlie Alzheimer's Disease. Pin1 binds to tau phosphorylated specifically on the Thr231-Pro site and induces conformational changes in tau. Such conformational changes can directly restore the ability of phosphorylated Tau to bind microtubules and promote microtubule assembly and/or facilitate tau dephosphorylation. Pin1 expression inversely correlates with the predicted neuronal vulnerability in normally aged brain and also with actual neurofibrillary degeneration in AD brain. Pin1 could be pivotal for maintainance of normal neuronal function and preventing age-dependent neurodegeneration.

Long Name

Protein [Peptidyl-prolyl cis/trans Isomerase] NIMA-interacting 1

Alternate Names

dod

Entrez Gene IDs

5300 (Human); 23988 (Mouse); 298696 (Rat)

Gene Symbol

PIN1

UniProt

NP_006212.1 (Human)

Additional Pin1 Products

Product Documents for Pin1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pin1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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