PIP Antibody (PIP/7477)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20296

Novus Biologicals, part of Bio-Techne
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NBP3-20296-100ug
NBP3-20296-20ug
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # PIP/7477

Concentration

0.2 mg/ml

View all PIP Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 41-146) of human PIP protein (exact sequence is proprietary)

Localization

Cytoplasm (Golgi bodies). Secreted.

Specificity

It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (PIP). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. PIP, also known as PIP, for prolactin inducible protein, is a prolactin and androgen-controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

Positive Controls: HepG2 cells. Breast or Pancreas.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for PIP Antibody (PIP/7477)

PIP Antibody (PIP/7477)

PIP Antibody (PIP/7477)

Formalin-fixed, paraffin-embedded human breast carcinoma stained with PIP antibody (PIP/7477). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for PIP Antibody (PIP/7477)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PIP

The androgen and prolactin responsive prolactin-inducible protein (PIP), also known as Gross cystic disease fluid protein (GCDFP-15) is expressed in benign and malignant human breast tumors and in such normal exocrine organs as sweat, salivary and lacrimal glands. The hormonally responsive PIP gene is expressed in benign and malignant breast tumor tissues and in such normal exocrine organs as sweat, salivary, and lacrimal glands. The cells within the body that produce PIP/GCDFP 15 appear to be restricted primarily to those with apocrine function. Studies have found PIP to be a highly specific and sensitive marker for breast cancer.

Alternate Names

GCDFP15prolactin-inducible protein, GCDFP-15SABP, GPIP4gp17, Gross cystic disease fluid protein 15, prolactin-induced proteinSecretory actin-binding protein

Gene Symbol

PIP

Additional PIP Products

Product Documents for PIP Antibody (PIP/7477)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PIP Antibody (PIP/7477)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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