Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (94%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PIWIL1/HIWI Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1. The immunogen is located within amino acids 60 - 110 of PIWI-L1. Amino Acid Squence: ELSLAERGGRRRDFHDLG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIWIL1/HIWI Antibody - BSA Free

Western Blot: PIWIL1/HIWI AntibodyBSA Free [NBP1-76489]

Western Blot: PIWIL1/HIWI AntibodyBSA Free [NBP1-76489]

Western Blot: PIWIL1/HIWI Antibody [NBP1-76489] - Analysis of PIWI-L1 in human testis tissue lysate with PIWI-L2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
PIWIL1/HIWI Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PIWIL1/HIWI Antibody - BSA Free [NBP1-76489] -

Immunocytochemistry/ Immunofluorescence: PIWIL1/HIWI Antibody - BSA Free [NBP1-76489] - Immunofluorescence of PIWIL1/HIWI in rat liver tissue with PIWIL1/HIWI antibody at 20 u/mL.
PIWIL1/HIWI Antibody - BSA Free

Immunohistochemistry: PIWIL1/HIWI Antibody - BSA Free [NBP1-76489] -

Immunohistochemistry: PIWIL1/HIWI Antibody - BSA Free [NBP1-76489] - Immunohistochemistry of PIWIL1/HIWI in rat liver tissue with PIWIL1/HIWI antibody at 10 u/mL.

Applications for PIWIL1/HIWI Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIWIL1/HIWI

PIWI-L1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L1 is thought to play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells as removing PIWI-L1 protein from single germline stem cells significantly decreases the rate of their division. Recent studies have shown that overexpression of PIWI-L1 (as well as other PIWI-like proteins) are potential biomarkers for colon and other cancers.

Long Name

Piwi-like Protein 1

Alternate Names

HIWI, MIWI

Gene Symbol

PIWIL1

UniProt

AAK69348 (Human)

Additional PIWIL1/HIWI Products

Product Documents for PIWIL1/HIWI Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PIWIL1/HIWI Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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