PKA RI beta Antibody (OTI2A3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00634
![Flow Cytometry: PKA RI beta Antibody (OTI2A3) [NBP2-00634]](https://resources.bio-techne.com/images/products/PKA-regulatory-subunit-I-beta-Antibody-2A3-Flow-Cytometry-NBP2-00634-img0003.jpg "Flow Cytometry: PKA RI beta Antibody (OTI2A3) [NBP2-00634]")
![Western Blot: PKA RI beta Antibody (OTI2A3) [NBP2-00634]](https://resources.bio-techne.com/images/products/PKA-regulatory-subunit-I-beta-Antibody-2A3-Western-Blot-NBP2-00634-img0004.jpg "Western Blot: PKA RI beta Antibody (OTI2A3) [NBP2-00634]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2A3
Concentration
0.57 mg/ml
Product Summary for PKA RI beta Antibody (OTI2A3)
Immunogen
Full length human recombinant protein of human PRKAR1B (NP_002726) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
42.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PKA RI beta Antibody (OTI2A3)
Western Blot: PKA RI beta Antibody (OTI2A3) [NBP2-00634]
Western Blot: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKA regulatory subunit I beta (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKA regulatory subunit I beta.![Immunocytochemistry/ Immunofluorescence: PKA RI beta Antibody (OTI2A3) [NBP2-00634]](https://resources.bio-techne.com/images/products/PKA-regulatory-subunit-I-beta-Antibody-2A3-Immunocytochemistry-Immunofluorescence-NBP2-00634-img0008.jpg "Immunocytochemistry/ Immunofluorescence: PKA RI beta Antibody (OTI2A3) [NBP2-00634]")
Immunocytochemistry/ Immunofluorescence: PKA RI beta Antibody (OTI2A3) [NBP2-00634]
Immunocytochemistry/Immunofluorescence: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKA regulatory subunit I beta.![Immunohistochemistry-Paraffin: PKA RI beta Antibody (OTI2A3) [NBP2-00634]](https://resources.bio-techne.com/images/products/PKA-regulatory-subunit-I-beta-Antibody-2A3-Immunohistochemistry-Paraffin-NBP2-00634-img0010.jpg "Immunohistochemistry-Paraffin: PKA RI beta Antibody (OTI2A3) [NBP2-00634]")
Immunohistochemistry-Paraffin: PKA RI beta Antibody (OTI2A3) [NBP2-00634]
Immunohistochemistry-Paraffin: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - Staining of paraffin-embedded Human lymphoma tissue using anti-PKA regulatory subunit I beta mouse monoclonal antibody.![Immunohistochemistry-Paraffin: PKA RI beta Antibody (OTI2A3) [NBP2-00634]](https://resources.bio-techne.com/images/products/PKA-regulatory-subunit-I-beta-Antibody-2A3-Immunohistochemistry-Paraffin-NBP2-00634-img0009.jpg "Immunohistochemistry-Paraffin: PKA RI beta Antibody (OTI2A3) [NBP2-00634]")
![Flow Cytometry: PKA RI beta Antibody (OTI2A3) [NBP2-00634]](https://resources.bio-techne.com/images/products/PKA-regulatory-subunit-I-beta-Antibody-2A3-Flow-Cytometry-NBP2-00634-img0001.jpg "Flow Cytometry: PKA RI beta Antibody (OTI2A3) [NBP2-00634]")
![Flow Cytometry: PKA RI beta Antibody (OTI2A3) [NBP2-00634]](https://resources.bio-techne.com/images/products/PKA-regulatory-subunit-I-beta-Antibody-2A3-Flow-Cytometry-NBP2-00634-img0002.jpg "Flow Cytometry: PKA RI beta Antibody (OTI2A3) [NBP2-00634]")
Applications for PKA RI beta Antibody (OTI2A3)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.57 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PKA RI beta
Long Name
cAMP-dependent Protein Kinase Regulatory Type I beta
Alternate Names
PRKAR1B, RIbeta
Entrez Gene IDs
5575 (Human)
Gene Symbol
PRKAR1B
Additional PKA RI beta Products
Product Documents for PKA RI beta Antibody (OTI2A3)
Product Specific Notices for PKA RI beta Antibody (OTI2A3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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