PKC alpha Antibody (MC5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80911
![Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911]](https://resources.bio-techne.com/images/products/PKC-alpha-Antibody-MC5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80911-img0001.jpg "Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Baboon, Bovine, Canine, Chicken, Fish, Monkey, Rabbit, Sheep, Zebrafish
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Radioimmunoassay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # MC5
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified bovine brain Protein Kinase C
Reactivity Notes
Chicken reactivity reported in scientific literature (PMID: 26719241).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for PKC alpha Antibody (MC5) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911]
Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911] - PKC alpha (MC5) antibody was tested in SH-SY5Y cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image from the standard format of this antibody.![Immunohistochemistry: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911]](https://resources.bio-techne.com/images/products/PKC-alpha-Antibody-MC5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80911-img0002.jpg "Immunohistochemistry: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911]")
Immunohistochemistry: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911]
Immunohistochemistry: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911] - IHC staining of PKC alpha in mouse brain using DAB with hematoxylin counterstain. Image from the standard format of this antibody.![Flow Cytometry: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911]](https://resources.bio-techne.com/images/products/PKC-alpha-Antibody-MC5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80911-img0003.jpg "Flow Cytometry: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911]")
Flow Cytometry: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911]
Flow Cytometry: PKC alpha Antibody (MC5) - Azide and BSA Free [NBP2-80911] - Detection of PKC alpha in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- PKC alpha Monoclonal Antibody (Catalog # NB600-201, filled histogram), or Mouse IgG2A isotype control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a Bio-Techne brand. Image from the standard format of this antibody.Applications for PKC alpha Antibody (MC5) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry
1:200-1:500
Immunohistochemistry-Frozen
1:200-1:500
Immunohistochemistry-Paraffin
1:200-1:500
Immunoprecipitation
1:10-1:100
Western Blot
1:1000
Application Notes
This PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Tris-Glycine, 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PKC alpha
Long Name
Protein Kinase C alpha
Alternate Names
PKCA, PRKCA
Gene Symbol
PRKCA
Additional PKC alpha Products
Product Documents for PKC alpha Antibody (MC5) - Azide and BSA Free
Product Specific Notices for PKC alpha Antibody (MC5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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