PKC alpha Antibody (MC5)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-201

Novus Biologicals, part of Bio-Techne
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NB600-201
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Baboon, Bovine, Canine, Chicken, Fish, Monkey, Rabbit, Sheep, Zebrafish

Cited:

Human, Mouse, Rat, Avian - Chicken, Fish - Danio rerio (Zebrafish), Primate - Macaca mulatta (Rhesus Macaque), Rabbit

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Radioimmunoassay, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # MC5

Concentration

1.0 mg/ml

View all PKC alpha Antibodies »

Product Specifications

Immunogen

Purified bovine brain Protein Kinase C

Reactivity Notes

Use in Monkey reported in scientific literature (PMID:35024589) Use in Baboon reported in scientific literature (PMID:34314739) Zebrafish reactivity reported in scientific literature (PMID: 31273575)..

Specificity

This antibody is capable of detecting PKC beta as well.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PKC alpha Antibody (MC5)

Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201]

Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201]

Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha (MC5) antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201]

Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201]

Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha detected in muse prostate tissue using PKC alpha Antibody (MC5). IHC-P image submitted by a verified customer review.
Flow Cytometry: PKC alpha Antibody (MC5) [NB600-201]

Flow Cytometry: PKC alpha Antibody (MC5) [NB600-201]

Flow Cytometry: PKC alpha Antibody (MC5) [NB600-201] - Detection of PKC alpha in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- PKC alpha Monoclonal Antibody (Catalog # NB600-201, filled histogram), or Mouse IgG2A isotype control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
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Applications for PKC alpha Antibody (MC5)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:200-1:500

Immunohistochemistry-Frozen

1:200-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Immunoprecipitation

1:10-1:100

Western Blot

1:1000
Application Notes
This PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha.

Reviewed Applications

Read 1 review rated 5 using NB600-201 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Tris-Glycine and 0.15 M NaCl

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC alpha

Protein Kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanisms. PKC alpha belongs to the conventional PKC group, alongside Beta-I, Beta-II, and Gamma, and is activated by phosphatidylserine in a calcium dependent manner. Furthermore, PKC alpha is localized to the membrane and cytoplasm, but in some cases can translocate to the nucleus. PKC alpha helps to positively and negatively regulate cell proliferation, apoptosis, differentiation, adhesion, and migration via phosphorylation of RAF1, BCL2, CSPG4, TNNT2/CTNT, or MAPK1/3. It also exhibits positive interactions with ADAP1/CENTA1, CSPG4 and PRKCABP. PKC alpha demonstrates anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3 (PMCID: PMC1360842). Additional research has shown PKC alpha's involvement in cellular inflammation (PMCID: PMC3080812).

Long Name

Protein Kinase C alpha

Alternate Names

PKCA, PRKCA

Entrez Gene IDs

5578 (Human); 75292 (Mouse); 313834 (Rat); 282001 (Bovine)

Gene Symbol

PRKCA

UniProt

P17252, P04409 (Bovine)

Additional PKC alpha Products

Product Documents for PKC alpha Antibody (MC5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PKC alpha Antibody (MC5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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