We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.

Name: PKC alpha Antibody
Brand: Novus Biologicals
SKU: NBP1-87269

We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

PKC alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87269

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CVINVPSLCGMDHTEKRGRIYLKAEVADEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRST

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC alpha Antibody

Western Blot: PKC alpha Antibody [NBP1-87269]

Western Blot: PKC alpha Antibody [NBP1-87269] - Analysis using Anti-PRKCA antibody NBP1-87269 (A) shows similar pattern to independent antibody NBP1-87268 (B).

Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody [NBP1-87269]

Immunocytochemistry/Immunofluorescence: PKC alpha Antibody [NBP1-87269] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol.

Immunohistochemistry-Paraffin: PKC alpha Antibody [NBP1-87269]

Immunohistochemistry-Paraffin: PKC alpha Antibody [NBP1-87269] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.

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Applications for PKC alpha Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC alpha

Protein Kinase C (PKC) is a large superfamily of serine/threonine kinases that mediate essential cellular signals required for activation, proliferation, differentiation and survival. There are at least ten PKC isotypes that are closely related in structure but that have distinct patterns of tissue distribution and function. The PKC isotypes can be subdivided into three classes based on primary structure and biochemical properties. These are: classical or conventional PKC isotypes (cPKC), novel PKC isotypes (nPKC) and atypical PKC isotypes (aPKC). All PKC isotypes share a characteristic equence motif C1 in addition to a serine/threonine-protein kinase domain. The cPKC isotypes include PKC

Long Name

Protein Kinase C alpha

Alternate Names

PKCA, PRKCA

Entrez Gene IDs

5578 (Human)

Gene Symbol

PRKCA

UniProt

P17252

Additional PKC alpha Products

Product Documents for PKC alpha Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for PKC alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

PKC alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87269

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all PKC alpha Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for PKC alpha Antibody

Western Blot: PKC alpha Antibody [NBP1-87269]

Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody [NBP1-87269]

Immunohistochemistry-Paraffin: PKC alpha Antibody [NBP1-87269]

Western Blot: PKC alpha Antibody [NBP1-87269]

Western Blot: PKC alpha Antibody [NBP1-87269]

Western Blot: PKC alpha Antibody [NBP1-87269]

Applications for PKC alpha Antibody

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: PKC alpha

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional PKC alpha Products

Product Documents for PKC alpha Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for PKC alpha Antibody