PKC alpha [p Ser319] Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05438
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic phosphorylated peptide around S319 of human PKC alpha (NP_002728.2). VISPS
Modification
p Ser319
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PKC alpha [p Ser319] Antibody - BSA Free
Western Blot: PKC alpha [p Ser319] Antibody [NBP3-05438] - Western blot analysis of extracts of HeLa cells, using PKC alpha antibody (NBP3-05438) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37C for 30 minutes after serum-starvation overnight.Hela cells were treated by ATP(5 mM) at 30C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Applications for PKC alpha [p Ser319] Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PKC alpha
Long Name
Protein Kinase C alpha
Alternate Names
PKCA, PRKCA
Gene Symbol
PRKCA
Additional PKC alpha Products
Product Documents for PKC alpha [p Ser319] Antibody - BSA Free
Product Specific Notices for PKC alpha [p Ser319] Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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