PKC alpha [p Ser657] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13311

Novus Biologicals, part of Bio-Techne
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NBP3-13311
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Chicken (100%), Mouse (100%), Rabbit (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKC alpha Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser657 of human PKC alpha. The exact sequence is proprietary.

Modification

p Ser 657

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PKC alpha [p Ser657] Antibody

Western Blot: PKC alpha [p Ser657] Antibody [NBP3-13311]

Western Blot: PKC alpha [p Ser657] Antibody [NBP3-13311]

Western Blot: PKC alpha [p Ser657] Antibody [NBP3-13311] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha (phospho Ser657) antibody (NBP3-13311) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: PKC alpha [p Ser657] Antibody [NBP3-13311]

Western Blot: PKC alpha [p Ser657] Antibody [NBP3-13311]

Western Blot: PKC alpha [p Ser657] Antibody [NBP3-13311] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha (phospho Ser657) antibody (NBP3-13311) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for PKC alpha [p Ser657] Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC alpha

Protein Kinase C (PKC) is a large superfamily of serine/threonine kinases that mediate essential cellular signals required for activation, proliferation, differentiation and survival. There are at least ten PKC isotypes that are closely related in structure but that have distinct patterns of tissue distribution and function. The PKC isotypes can be subdivided into three classes based on primary structure and biochemical properties. These are: classical or conventional PKC isotypes (cPKC), novel PKC isotypes (nPKC) and atypical PKC isotypes (aPKC). All PKC isotypes share a characteristic equence motif C1 in addition to a serine/threonine-protein kinase domain. The cPKC isotypes include PKC

Long Name

Protein Kinase C alpha

Alternate Names

PKCA, PRKCA

Gene Symbol

PRKCA

Additional PKC alpha Products

Product Documents for PKC alpha [p Ser657] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PKC alpha [p Ser657] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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