PKC delta [p Ser645] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82141

Novus Biologicals, part of Bio-Techne
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NB100-82141
NB100-82141-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PKC delta Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human PKC delta around the phosphorylation site of serine 645 (R-L-SP-Y-S).

Modification

p Ser645

Specificity

Detects endogenous levels of PKC delta only when phosphorylated at serine 645.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKC delta [p Ser645] Antibody

Immunohistochemistry-Paraffin: PKC delta [p Ser645] Antibody [NB100-82141] -

Immunohistochemistry-Paraffin: PKC delta [p Ser645] Antibody [NB100-82141] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82141 (left) or the same antibody preincubated with blocking peptide(right).
Western Blot: PKC delta [p Ser645] Antibody [NB100-82141]

Western Blot: PKC delta [p Ser645] Antibody [NB100-82141]

Western Blot: PKC delta [p Ser645] Antibody [NB100-82141] - Analysis of extracts from PC12 cells untreated or treated with PMA using PKCd(Phospho-Ser645) antibody.

Applications for PKC delta [p Ser645] Antibody

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC delta

Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.

Long Name

Protein Kinase C delta

Alternate Names

PRKCD, pPKCY

Entrez Gene IDs

5580 (Human); 170538 (Rat)

Gene Symbol

PRKCD

UniProt

Q05655

Additional PKC delta Products

Product Documents for PKC delta [p Ser645] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PKC delta [p Ser645] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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