PKC epsilon [p Ser729] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13312

Novus Biologicals, part of Bio-Techne
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NBP3-13312
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Rabbit (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKC epsilon Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser729 of human PKC epsilon. The exact sequence is proprietary.

Modification

p Ser729

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PKC epsilon [p Ser729] Antibody

Western Blot: PKC epsilon [p Ser729] Antibody [NBP3-13312]

Western Blot: PKC epsilon [p Ser729] Antibody [NBP3-13312]

Western Blot: PKC epsilon [p Ser729] Antibody [NBP3-13312] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC epsilon (phospho Ser729) antibody (NBP3-13312) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PKC epsilon [p Ser729] Antibody [NBP3-13312]

Immunohistochemistry-Paraffin: PKC epsilon [p Ser729] Antibody [NBP3-13312]

Immunohistochemistry-Paraffin: PKC epsilon [p Ser729] Antibody [NBP3-13312] - Rat brain. PKC epsilon (phospho Ser729) stained by PKC epsilon (phospho Ser729) antibody (NBP3-13312) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: PKC epsilon [p Ser729] Antibody [NBP3-13312]

Western Blot: PKC epsilon [p Ser729] Antibody [NBP3-13312]

Western Blot: PKC epsilon [p Ser729] Antibody [NBP3-13312] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC epsilon (phospho Ser729) antibody (NBP3-13312) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
View 1 more images

Applications for PKC epsilon [p Ser729] Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC epsilon

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.

Long Name

Protein Kinase C epsilon

Alternate Names

PKCE, PRKCE

Gene Symbol

PRKCE

Additional PKC epsilon Products

Product Documents for PKC epsilon [p Ser729] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PKC epsilon [p Ser729] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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