PKC iota Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84959

Novus Biologicals, part of Bio-Techne
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NBP1-84959
NBP1-84959-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (93%), Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKC iota Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: INCKLLVHKKCHKLVTIECGRHSLPQEPVMPMDQSSMHSDHAQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC iota Antibody

Western Blot: PKC iota Antibody [NBP1-84959]

Western Blot: PKC iota Antibody [NBP1-84959]

Western Blot: PKC iota Antibody [NBP1-84959] - Analysis in human cell line A-431.
Immunocytochemistry/ Immunofluorescence: PKC iota Antibody [NBP1-84959]

Immunocytochemistry/ Immunofluorescence: PKC iota Antibody [NBP1-84959]

Immunocytochemistry/Immunofluorescence: PKC iota Antibody [NBP1-84959] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PKC iota Antibody [NBP1-84959]

Immunohistochemistry-Paraffin: PKC iota Antibody [NBP1-84959]

Immunohistochemistry-Paraffin: PKC iota Antibody [NBP1-84959] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.
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Applications for PKC iota Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC iota

PKC iota encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.

Long Name

Protein kinase C iota type

Alternate Names

aPKC-lambda/iota, DXS1179E, nPKC-iota, PRKC-lambda/iota

Gene Symbol

PRKCI

Additional PKC iota Products

Product Documents for PKC iota Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKC iota Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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