PKC theta Antibody (1B2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15185

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15185
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1B2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKC theta Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human PKC theta. UniProt ID (Q04759).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC theta Antibody (1B2)

Western Blot: PKC theta Antibody (1B2) [NBP3-15185]

Western Blot: PKC theta Antibody (1B2) [NBP3-15185]

Western Blot: PKC theta Antibody (1B2) [NBP3-15185] - Positive WB detected in: 293T whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate, Mouse Brain whole cell lysate. All lanes: PKC theta antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 82, 75, 68 KDa. Observed band size: 82, 55 KDa.

Applications for PKC theta Antibody (1B2)

Application
Recommended Usage

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: PKC theta

Members of the protein kinase C (PKC) family play a key regulatory role in variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, episilon , zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and episilon are independent of Ca++. On the other hand, nPKC and episilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

Long Name

Protein Kinase C theta

Alternate Names

PRKCQ, PRKCT

Gene Symbol

PRKCQ

Additional PKC theta Products

Product Documents for PKC theta Antibody (1B2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKC theta Antibody (1B2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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