PKC theta [p Ser676] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82145

Novus Biologicals, part of Bio-Techne
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NB100-82145
NB100-82145-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PKC theta Antibodies »

Product Specifications

Immunogen

Peptide sequence around phosphorylation site of serine 676 (R-L-S(p)-F-A) derived from Human PKC theta.

Modification

p Ser676

Specificity

Detects endogenous levels of PKC theta only when phosphorylated at serine 676.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKC theta [p Ser676] Antibody

Western Blot: PKC theta [p Ser676] Antibody [NB100-82145] - Analysis of extracts from PC12 cells, treated with PMA or calf intestinal phosphatase (CIP), usingPKC theta [p Ser676] Antibody.

Immunocytochemistry/Immunofluorescence: PKC theta [p Ser676] Antibody [NB100-82145] -

Immunocytochemistry/Immunofluorescence: PKC theta [p Ser676] Antibody [NB100-82145] - Staining of methanol-fixed Hela cells using NB100-82145

Immunohistochemistry-Paraffin: PKC theta [p Ser676] Antibody [NB100-82145] -

Immunohistochemistry-Paraffin: PKC theta [p Ser676] Antibody [NB100-82145] - Staining of human lung carcinoma tissue, using NB100-82145 (left) or the same antibody preincubated with blocking peptide(right).
View 1 more images

Applications for PKC theta [p Ser676] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC theta

Protein kinase C (PKC) is a family of serine/threonine kinases that plays crucial roles in signal transduction. Members of the PKC family are divided into three groups based on their molecular structures and activating mechanisms: 1) Conventional PKC (alpha, beta, and gamma) requiring calcium, phosphatidylserine (PS), and diacylglycerol (DG) for activation; 2) novel PKC (sigma, epsilon, eta, and theta) activated independent of calcium; and 3) Atypical PKC (zeta and lamda), which are independent of both calcium and DG. High-level expression of PKC-theta was found in skeletal muscle, lung, T cells, and brain, and minimal expression in cardiac muscle, placenta, and liver. PKC theta is a autophosphorylated protein kinase.

Long Name

Protein Kinase C theta

Alternate Names

PRKCQ, PRKCT

Entrez Gene IDs

5588 (Human); 18761 (Mouse); 85420 (Rat)

Gene Symbol

PRKCQ

UniProt

Q04759

Additional PKC theta Products

Product Documents for PKC theta [p Ser676] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PKC theta [p Ser676] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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