PKC theta Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29867

Novus Biologicals, part of Bio-Techne
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NBP3-29867
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKC theta Antibodies »

Product Specifications

Immunogen

Between 656 and 706

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC theta Antibody - BSA Free

PKC theta Antibody

Immunoprecipitation:[NBP3-29867]-

Immunoprecipitation:[NBP3-29867]-Detection of human PRKCQ by western blot of immunoprecipitates. Samples: Whole cell lysate from Jurkat cells. Antibodies: Affinity purified rabbit anti-PRKCQ antibody used for IP at 6 µg/mg lysate. PRKCQ was also immunoprecipitated by rabbit anti-PRKCQ antibody. For blotting immunoprecipitated PRKCQ, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for PKC theta Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PKC theta

Members of the protein kinase C (PKC) family play a key regulatory role in variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, episilon , zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and episilon are independent of Ca++. On the other hand, nPKC and episilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

Long Name

Protein Kinase C theta

Alternate Names

PRKCQ, PRKCT

Gene Symbol

PRKCQ

Additional PKC theta Products

Product Documents for PKC theta Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PKC theta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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