PKC zeta Antibody (2D1)

Novus Biologicals, part of Bio-Techne | Catalog # H00005590-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2D1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKC zeta Antibodies »

Product Specifications

Immunogen

PRKCZ (AAH08058, 165 a.a. ~ 255 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KLLVHKRCHGLVPLTCRKHMDSVMPSQEPPVDDKNEDADLPSEETDGIAYISSSRKHDSIKDDSEDLKPVIDGMDGIKISQGLGLQDFDLI

Localization

Cytoplasmic and endosome

Specificity

PRKCZ - protein kinase C, zeta

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PKC zeta Antibody (2D1)

Western Blot: PKC zeta Antibody (2D1) [H00005590-M01]

Western Blot: PKC zeta Antibody (2D1) [H00005590-M01]

Western Blot: PKC zeta Antibody (2D1) [H00005590-M01] - Analysis of PRKCZ expression in transfected 293T cell line by PRKCZ monoclonal antibody (M01), clone 2D1.Lane 1: PRKCZ transfected lysate(67.7 KDa).Lane 2: Non-transfected lysate.
Western Blot: PKC zeta Antibody (2D1) [H00005590-M01]

Western Blot: PKC zeta Antibody (2D1) [H00005590-M01]

Western Blot: PKC zeta Antibody (2D1) [H00005590-M01] - Analysis of PRKCZ over-expressed 293 cell line, cotransfected with PRKCZ Validated Chimera RNAi ( Cat # H00005590-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCZ monoclonal antibody (M01) clone 2D1 (Cat # H00005590-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: PKC zeta Antibody (2D1) [H00005590-M01] - Detection limit for recombinant GST tagged PRKCZ is approximately 0.1ng/ml as a capture antibody.

Applications for PKC zeta Antibody (2D1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKC zeta

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.

Long Name

Protein Kinase C zeta

Alternate Names

PKC2, PRKCZ

Entrez Gene IDs

5590 (Human)

Gene Symbol

PRKCZ

OMIM

176982 (Human)

UniProt

AAH08058 (Human)

Additional PKC zeta Products

Product Documents for PKC zeta Antibody (2D1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKC zeta Antibody (2D1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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