PKM2 Antibody (GT1810)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13632

Novus Biologicals, part of Bio-Techne
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NBP3-13632
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (97%), Rabbit (97%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT1810

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKM2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKM2. The exact sequence is proprietary.

Reactivity Notes

Chicken (83%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PKM2 Antibody (GT1810)

Western Blot: PKM2 Antibody (GT1810) [NBP3-13632]

Western Blot: PKM2 Antibody (GT1810) [NBP3-13632]

Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: PKM2 Antibody (GT1810) [NBP3-13632]

Immunocytochemistry/ Immunofluorescence: PKM2 Antibody (GT1810) [NBP3-13632]

Immunocytochemistry/Immunofluorescence: PKM2 Antibody (GT1810) [NBP3-13632] - PKM2 antibody [GT1810] detects PKM2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKM2 stained by PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.
Immunohistochemistry-Paraffin: PKM2 Antibody (GT1810) [NBP3-13632]

Immunohistochemistry-Paraffin: PKM2 Antibody (GT1810) [NBP3-13632]

Immunohistochemistry-Paraffin: PKM2 Antibody (GT1810) [NBP3-13632] - Human lung cancer. PKM2 stained by PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for PKM2 Antibody (GT1810)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000
Application Notes
IHC-P-Assay dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKM2

PKM2 is encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Long Name

Pyruvate Kinase, Muscle

Alternate Names

CTHBP, OIP3, p58, PK2, PK3, TCB, THBP1, Tumor M2-PK

Gene Symbol

PKM

Additional PKM2 Products

Product Documents for PKM2 Antibody (GT1810)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKM2 Antibody (GT1810)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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