PKM2 Antibody (SR2031)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21688

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21688-100ul
NBP3-21688-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2031

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKM2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human PKM2 (Uniprot #: P14618)

Specificity

Detects endogenous levels of PKM2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKM2 Antibody (SR2031)

Western Blot: PKM2 Antibody (SR2031) [NBP3-21688] -

Western Blot: PKM2 Antibody (SR2031) [NBP3-21688] - Western blot analysis using NBP3-21688 in HeLa cell lysate.

Immunohistochemistry: PKM2 Antibody (SR2031) [NBP3-21688] -

Immunohistochemistry: PKM2 Antibody (SR2031) [NBP3-21688] - Immunohistochemical analysis of paraffin-embedded human cervix cancer, using NBP3-21688.

Applications for PKM2 Antibody (SR2031)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKM2

PKM2 is encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Long Name

Pyruvate Kinase, Muscle

Alternate Names

CTHBP, OIP3, p58, PK2, PK3, TCB, THBP1, Tumor M2-PK

Gene Symbol

PKM

Additional PKM2 Products

Product Documents for PKM2 Antibody (SR2031)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKM2 Antibody (SR2031)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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