PKR [p Thr446] Antibody (SY230)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67426
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
Chromatin Immunoprecipitation, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SY230
Concentration
1 mg/ml
Product Summary for PKR [p Thr446] Antibody (SY230)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr446 of Human PKR aa 421-470 / 551. (SwissProt: P19525 Human)
Modification
p Thr446
Localization
Cytoplasm, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PKR [p Thr446] Antibody (SY230)
Western Blot: PKR [p Thr446] Antibody (SY230) [NBP2-67426] - Analysis of Phospho-PKR(T446) on different lysates using anti-Phospho-PKR(T446) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela treated with Calyculin A and TNF-alpha whole cell lysates Lane 2: Untreated Hela whole cell lysates
Immunohistochemistry-Paraffin: PKR [p Thr446] Antibody (SY230) [NBP2-67426] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: PKR [p Thr446] Antibody (SY230) [NBP2-67426] - Analysis of paraffin-embedded human kidney tissue using anti-Phospho-PKR(T446) antibody. Counter stained with hematoxylin.
Applications for PKR [p Thr446] Antibody (SY230)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PKR
Long Name
Protein Kinase R
Alternate Names
EIF2AK2, P68 Kinase, PPP1R83, PRKR, Protein Kinase R
Gene Symbol
EIF2AK2
Additional PKR Products
Product Documents for PKR [p Thr446] Antibody (SY230)
Product Specific Notices for PKR [p Thr446] Antibody (SY230)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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