PLAP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84996

Novus Biologicals, part of Bio-Techne
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NBP1-84996
NBP1-84996-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PLAP Antibodies »

Product Summary for PLAP Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EGKEFDYVFSIDVNEGGPSYKLPYNTSDDPWLTAYNFLQKNDLNPMFLDQVAKFIIDNTKGQMLGLGNPSFSDPFTGGGRYVPGSSGSSNTLPTADP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLAP Antibody

Western Blot: PLAP Antibody [NBP1-84996]

Western Blot: PLAP Antibody [NBP1-84996]

Western Blot: PLAP Antibody [NBP1-84996] - Analysis using Anti-PLAA antibody NBP1-84996 (A) shows similar pattern to independent antibody NBP1-84997 (B).
Immunocytochemistry/ Immunofluorescence: PLAP Antibody [NBP1-84996]

Immunocytochemistry/ Immunofluorescence: PLAP Antibody [NBP1-84996]

Immunocytochemistry/Immunofluorescence: PLAP Antibody [NBP1-84996] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: PLAP Antibody [NBP1-84996]

Immunohistochemistry-Paraffin: PLAP Antibody [NBP1-84996]

Immunohistochemistry-Paraffin: PLAP Antibody [NBP1-84996] - Staining of human testis shows strong positivity in a subset of cells in seminiferus ducts.
View 1 more images

Applications for PLAP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000-1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLAP

Phospholipase A2 activator protein (PLAP), activates PLA2 (phospholipase A2 enzyme) and is an important mediator of eicosanoid generation. Also known as a pro-inflammatory agent, PLAP has been found in inflamed tissues and synovial fluid from patients with rheumatoid arthritis. As such, it is believed to play an important role in the regulation of inflammatory diseases. The formation of PLAP can be stimulated by IL-1 beta and TNF-alpha (1-2).

Alternate Names

DOA1, FLJ12699, phospholipase A-2-activating protein, phospholipase A2-activating protein, PLAP

Gene Symbol

PLAA

Additional PLAP Products

Product Documents for PLAP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PLAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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