PLC-gamma 2 Antibody (3A8B6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52534

Novus Biologicals, part of Bio-Techne
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NBP2-52534
NBP2-52534-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 3A8B6

Format

BSA Free

Concentration

1 mg/ml

View all PLC-gamma 2 Antibodies »

Product Summary for PLC-gamma 2 Antibody (3A8B6) - BSA Free

Immunogen

Synthesized peptide of human PLC-gamma 2 (AA: phospho-Tyrosine 753 of human Phospholipase Cg2 (cERDINSLpYDVSRMYV)).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

148 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLC-gamma 2 Antibody (3A8B6) - BSA Free

Western Blot: PLC-gamma 2 Antibody (3A8B6)BSA Free [NBP2-52534]

Western Blot: PLC-gamma 2 Antibody (3A8B6)BSA Free [NBP2-52534]

Western Blot: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis using PLCG2 mouse mAb against A431 cell lysate.
Immunocytochemistry/ Immunofluorescence: PLC-gamma 2 Antibody (3A8B6) - BSA Free [NBP2-52534]

Immunocytochemistry/ Immunofluorescence: PLC-gamma 2 Antibody (3A8B6) - BSA Free [NBP2-52534]

Immunocytochemistry/Immunofluorescence: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Immunofluorescence analysis of Hela cells using PLCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: PLC-gamma 2 Antibody (3A8B6) - BSA Free [NBP2-52534]

Flow Cytometry: PLC-gamma 2 Antibody (3A8B6) - BSA Free [NBP2-52534]

Flow Cytometry: PLC-gamma 2 Antibody (3A8B6) [NBP2-52534] - Analysis of Hela cells using PLCG2 mouse mAb (green) and negative control (red).
View 1 more images

Applications for PLC-gamma 2 Antibody (3A8B6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLC-gamma 2

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. The phospholipase C gamma-2 (PLCgammaII) is an enzyme that plays a crucial role in intracellular signal transduction pathways. This enzyme is important because of its role in the generation of second messengers following the hydrolysis of phosphatidylinositol 4,5-bisphosphate (1). PLCgammaII has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLCgammaII (2). It has also been shown that PLCgammaII was required for receptor activator of NF-kappaB ligand-induced (RANKL-induced) osteoclastogenesis by differentially regulating nuclear factor of activated T cells c1 (NFATc1), activator protein-1 (AP1), and NF-kappaB (3).

Long Name

Phospholipase C gamma 2

Alternate Names

PLCG2, PLCgamma 2

Gene Symbol

PLCG2

Additional PLC-gamma 2 Products

Product Documents for PLC-gamma 2 Antibody (3A8B6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PLC-gamma 2 Antibody (3A8B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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