PLCD4 Antibody (4D4)

Novus Biologicals, part of Bio-Techne | Catalog # H00084812-M01

Novus Biologicals, part of Bio-Techne
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H00084812-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4D4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PLCD4 Antibodies »

Product Specifications

Immunogen

PLCD4 (AAH06355, 18 a.a. ~ 127 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQEGMPMRKVRSKSWKKLRYFRLQNDGMTVWHARQARGSAKPSFSISDVETIRNGHDSELLRSLAEELPLEQGFTIVFHGRRSNLDLMANSVEEAQIWMRGLQLLVDLVT

Reactivity Notes

Human. Other species not tested.

Localization

Membrane; Peripheral membrane protein. Nucleus. Cytoplasm. Endoplasmic reticulum. Note=Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.

Specificity

PLCD4 - phospholipase C, delta 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for PLCD4 Antibody (4D4)

Western Blot: PLCD4 Antibody (4D4) [H00084812-M01]

Western Blot: PLCD4 Antibody (4D4) [H00084812-M01]

Western Blot: PLCD4 Antibody (4D4) [H00084812-M01] - Analysis of PLCD4 expression in transfected 293T cell line by PLCD4 monoclonal antibody (M01), clone 4D4.Lane 1: PLCD4 transfected lysate (Predicted MW: 87.6 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: PLCD4 Antibody (4D4) [H00084812-M01] - Detection limit for recombinant GST tagged PLCD4 is approximately 0.3ng/ml as a capture antibody.

Applications for PLCD4 Antibody (4D4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PLCD4

Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression upregulates the Erk signaling pathway and proliferation.

Alternate Names

EC 3.1.4.11, hPLCD4, MGC12837, Phosphoinositide phospholipase C-delta-4, phospholipase C, delta 4, Phospholipase C-delta-4, PLC delta4, PLC-delta-4,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-4

Entrez Gene IDs

84812 (Human)

Gene Symbol

PLCD4

OMIM

605939 (Human)

UniProt

AAH06355 (Human)

Additional PLCD4 Products

Product Documents for PLCD4 Antibody (4D4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PLCD4 Antibody (4D4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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