Pleckstrin Antibody (2D8)
Novus Biologicals, part of Bio-Techne | Catalog # H00005341-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2D8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PLEK (AAH18549, 121 a.a. ~ 230 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PETIDLGALYLSMKDTEKGIKELNLEKDKKIFNHCFTGNCVIDWLVSNQSVRNRQEGLMIASSLLNEGYLQPAGDMSKSAVDGTAENPFLDNPDAFYYFPDSGFFCEENS
Specificity
PLEK - pleckstrin (2D8)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Pleckstrin Antibody (2D8)
![Western Blot: Pleckstrin Antibody (2D8) [H00005341-M03]](https://resources.bio-techne.com/images/products/Pleckstrin-Antibody-2D8-Western-Blot-H00005341-M03-img0006.jpg "Western Blot: Pleckstrin Antibody (2D8) [H00005341-M03]")
Western Blot: Pleckstrin Antibody (2D8) [H00005341-M03]
Western Blot: Pleckstrin Antibody (2D8) [H00005341-M03] - Analysis of PLEK expression in HL-60 (Cat # L014V1).![Immunocytochemistry/ Immunofluorescence: Pleckstrin Antibody (2D8) [H00005341-M03]](https://resources.bio-techne.com/images/products/Pleckstrin-Antibody-2D8-Immunocytochemistry-Immunofluorescence-H00005341-M03-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Pleckstrin Antibody (2D8) [H00005341-M03]")
Immunocytochemistry/ Immunofluorescence: Pleckstrin Antibody (2D8) [H00005341-M03]
Immunocytochemistry/Immunofluorescence: Pleckstrin Antibody (2D8) [H00005341-M03] - Analysis of monoclonal antibody to PLEK on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Pleckstrin Antibody (2D8) [H00005341-M03] - Detection limit for recombinant GST tagged PLEK is approximately 3ng/ml as a capture antibody.
Applications for Pleckstrin Antibody (2D8)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Pleckstrin
Alternate Names
FLJ27168, p47, P47Platelet 47 kDa protein, pleckstrin
Entrez Gene IDs
5341 (Human)
Gene Symbol
PLEK
UniProt
Additional Pleckstrin Products
Product Documents for Pleckstrin Antibody (2D8)
Product Specific Notices for Pleckstrin Antibody (2D8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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