Pleckstrin Antibody (OTI6H8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02300
![Immunohistochemistry-Paraffin: Pleckstrin Antibody (OTI6H8) [NBP2-02300]](https://resources.bio-techne.com/images/products/Pleckstrin-Antibody-6H8-Immunohistochemistry-Paraffin-NBP2-02300-img0003.jpg "Immunohistochemistry-Paraffin: Pleckstrin Antibody (OTI6H8) [NBP2-02300]")
![Western Blot: Pleckstrin Antibody (OTI6H8) [NBP2-02300]](https://resources.bio-techne.com/images/products/Pleckstrin-Antibody-6H8-Western-Blot-NBP2-02300-img0002.jpg "Western Blot: Pleckstrin Antibody (OTI6H8) [NBP2-02300]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI6H8
Concentration
0.82 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human PLEK (NP_002655) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
40.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Pleckstrin Antibody (OTI6H8)
Western Blot: Pleckstrin Antibody (OTI6H8) [NBP2-02300]
Western Blot: Pleckstrin Antibody (6H8) [NBP2-02300] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Pleckstrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Pleckstrin.
Immunohistochemistry-Paraffin: Pleckstrin Antibody (6H8) [NBP2-02300] Staining of paraffin-embedded prostate tissue using anti-Pleckstrin mouse monoclonal antibody.
![Flow Cytometry: Pleckstrin Antibody (OTI6H8) [NBP2-02300]](https://resources.bio-techne.com/images/products/Pleckstrin-Antibody-6H8-Flow-Cytometry-NBP2-02300-img0001.jpg "Flow Cytometry: Pleckstrin Antibody (OTI6H8) [NBP2-02300]")
Flow Cytometry: Pleckstrin Antibody (OTI6H8) [NBP2-02300]
Flow Cytometry: Pleckstrin Antibody (6H8) [NBP2-02300] - HEK293T cells transfected with either pCMV6-ENTRY Pleckstrin.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Pleckstrin mouse monoclonal, and then analyzed by flow cytometry.![Immunohistochemistry-Paraffin: Pleckstrin Antibody (OTI6H8) [NBP2-02300]](https://resources.bio-techne.com/images/products/Pleckstrin-Antibody-6H8-Immunohistochemistry-Paraffin-NBP2-02300-img0004.jpg "Immunohistochemistry-Paraffin: Pleckstrin Antibody (OTI6H8) [NBP2-02300]")
![Immunohistochemistry-Paraffin: Pleckstrin Antibody (OTI6H8) [NBP2-02300]](https://resources.bio-techne.com/images/products/Pleckstrin-Antibody-6H8-Immunohistochemistry-Paraffin-NBP2-02300-img0005.jpg "Immunohistochemistry-Paraffin: Pleckstrin Antibody (OTI6H8) [NBP2-02300]")
![Immunohistochemistry-Paraffin: Pleckstrin Antibody (OTI6H8) [NBP2-02300]](https://resources.bio-techne.com/images/products/Pleckstrin-Antibody-6H8-Immunohistochemistry-Paraffin-NBP2-02300-img0006.jpg "Immunohistochemistry-Paraffin: Pleckstrin Antibody (OTI6H8) [NBP2-02300]")
Applications for Pleckstrin Antibody (OTI6H8)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:50
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.82 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Pleckstrin
Alternate Names
FLJ27168, p47, P47Platelet 47 kDa protein, pleckstrin
Entrez Gene IDs
5341 (Human)
Gene Symbol
PLEK
Additional Pleckstrin Products
Product Documents for Pleckstrin Antibody (OTI6H8)
Product Specific Notices for Pleckstrin Antibody (OTI6H8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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