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Plectin Antibody (4D12)

Novus Biologicals, part of Bio-Techne | Catalog # H00005339-M02

Novus Biologicals, part of Bio-Techne
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H00005339-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4D12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PLEC1 (NP_000436, 4384 a.a. ~ 4493 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CGFEDPRTKTKMSAAQALKKGWLYYEAGQRFLEVQYLTGGLIEPDTPGRVPLDEALQRGTVDARTAQKLRDVGAYSKYLTCPKTKLKISYKDALDRSMVEEGTGLRLLEA

Specificity

PLEC1 (4D12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Plectin Antibody (4D12)

Sandwich ELISA: Plectin Antibody (4D12) [H00005339-M02] - Detection limit for recombinant GST tagged PLEC1 is approximately 0.3ng/ml as a capture antibody.

Applications for Plectin Antibody (4D12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Plectin

The product of this gene belongs to the plakin or cytolinker family of genes. The encoded protein is involved in cytoskeleton-membrane attachment in epithelial cells, muscle, and other tissues. It also may regulate changes of lymphocyte cytoarchitecture during polarization and extravasation. This gene has alternative splicing of different 5' exons to the first constant exon, which generates transcript variants that encode distinct isoforms.

Alternate Names

EBS1, HD1, Hemidesmosomal protein 1, LGMD2Q, PCNplectin 1, intermediate filament binding protein, 500kD, PLEC1b, PLEC1EBSO, plectin, plectin 1, intermediate filament binding protein 500kDa, plectin-1, PLTNepidermolysis bullosa simplex 1 (Ogna)

Entrez Gene IDs

5339 (Human)

Gene Symbol

PLEC

OMIM

131950 (Human)

Additional Plectin Products

Product Documents for Plectin Antibody (4D12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Plectin Antibody (4D12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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