Plectin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12950
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Rat (90%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Plectin. The exact sequence is proprietary.
Reactivity Notes
Mouse (87%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Plectin Antibody
![Western Blot: Plectin Antibody [NBP3-12950]](https://resources.bio-techne.com/images/products/Plectin-Antibody-Western-Blot-NBP3-12950-img0001.jpg "Western Blot: Plectin Antibody [NBP3-12950]")
Western Blot: Plectin Antibody [NBP3-12950]
Western Blot: Plectin Antibody [NBP3-12950] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Plectin antibody (NBP3-12950) diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: Plectin Antibody [NBP3-12950] -
Immunocytochemistry/ Immunofluorescence: Plectin Antibody [NBP3-12950] - Plectin antibody detects Plectin protein at cytoskeleton by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Plectin stained by Plectin antibody (NBP3-12950) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.Applications for Plectin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockout Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Plectin
Alternate Names
EBS1, HD1, Hemidesmosomal protein 1, LGMD2Q, PCNplectin 1, intermediate filament binding protein, 500kD, PLEC1b, PLEC1EBSO, plectin, plectin 1, intermediate filament binding protein 500kDa, plectin-1, PLTNepidermolysis bullosa simplex 1 (Ogna)
Gene Symbol
PLEC
Additional Plectin Products
Product Specific Notices for Plectin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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