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PLEKHA3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32018

Novus Biologicals, part of Bio-Techne
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NBP2-32018
NBP2-32018-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GSCSSERSSHSIKEPVSTLHRLSQRRRRTYSDTDSCSDIPLEDPDRPVHCSKNTLNGDLASATIPEESRLMAKKQSESEDTLPSFSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLEKHA3 Antibody

Western Blot: PLEKHA3 Antibody [NBP2-32018]

Western Blot: PLEKHA3 Antibody [NBP2-32018]

Western Blot: PLEKHA3 Antibody [NBP2-32018] - Analysis using Anti-PLEKHA3 antibody NBP2-32018 (A) shows similar pattern to independent antibody NBP1-83924 (B).
Immunocytochemistry/ Immunofluorescence: PLEKHA3 Antibody [NBP2-32018]

Immunocytochemistry/ Immunofluorescence: PLEKHA3 Antibody [NBP2-32018]

Immunocytochemistry/Immunofluorescence: PLEKHA3 Antibody [NBP2-32018] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PLEKHA3 Antibody [NBP2-32018]

Immunohistochemistry-Paraffin: PLEKHA3 Antibody [NBP2-32018]

Immunohistochemistry-Paraffin: PLEKHA3 Antibody [NBP2-32018] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Applications for PLEKHA3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLEKHA3

PLEKHA3 binds specifically to phosphatidylinositol-4-phosphate (PtdIns4P), but not to other phosphoinositides

Alternate Names

FAPP-1, FAPP1PH domain-containing family A member 3, FLJ20067, four-phosphate-adaptor protein 1, Phosphatidylinositol-four-phosphate adapter protein 1, Phosphoinositol 4-phosphate adapter protein 1, pleckstrin homology domain containing, family A (phosphoinositide bindingspecific) member 3, pleckstrin homology domain-containing family A member 3, pleckstrin homology domain-containing, family A (phosphoinositide bindingspecific) member 3

Gene Symbol

PLEKHA3

UniProt

Additional PLEKHA3 Products

Product Documents for PLEKHA3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PLEKHA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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