Plexin D1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-961

Novus Biologicals, part of Bio-Techne
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NB300-961
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Plexin D1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-LAEPKKSHRQSH corresponding to internal region according to NP_055918.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Plexin D1 Antibody

Immunocytochemistry/ Immunofluorescence: Plexin D1 Antibody [NB300-961]

Immunocytochemistry/ Immunofluorescence: Plexin D1 Antibody [NB300-961]

Immunocytochemistry/Immunofluorescence: Plexin D1 Antibody [NB300-961] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: Plexin D1 Antibody [NB300-961]

Flow Cytometry: Plexin D1 Antibody [NB300-961]

Flow Cytometry: Plexin D1 Antibody [NB300-961] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: Plexin D1 Antibody [NB300-961]

Immunocytochemistry/ Immunofluorescence: Plexin D1 Antibody [NB300-961]

Immunocytochemistry/Immunofluorescence: Plexin D1 Antibody [NB300-961] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for Plexin D1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:16000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Plexin D1

Plexin D1 is a type I transmembrane glycoprotein that is the prototype of the plexin D subfamily of semaphorin receptors. Human Plexin D1 contains a 46 amino acid (aa) signal sequence, a 1225 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 633 aa cytoplasmic domain that includes features common to other plexins. The human Plexin D1 ECD shares 89% identity with mouse Plexin D1, and approximately 84-92% aa identity based on incomplete sequences of rat, bovine, porcine and canine Plexin D1. It contains a sema domain, two plexin-semaphorin-integrin (PSI) or Met-related sequence (MRS) cysteine-rich motifs, and three glycine/proline-rich IPT/TIG domains which are immunoglobulin-like domains found in plexins, transcription factors, and the scatter factor receptors Met and Ron. Isoforms of 1787 and 1747 aa have been sequenced; these contain a 178 aa N-terminal deletion with or without a longer alternate C-terminus. Like other Sema/plexin interactions, Plexin D1 interacts with Sema3C or Sema4A via neuropilins. Interaction with Sema3E, however, is direct. Plexin D1/Sema3E interaction mediates vascular guidance during development or angiogenesis; deletion of either molecule results in similar, profound cardiac abnormalities. Plexin D1 is also expressed in lymphocytes, osteoblasts, the neural crest and the central nervous system during development. In the brain, the presence of neuropilin can change Plexin D1/Sema3E interaction from an attractive to a repulsive signal. Plexin D1 directs migration of thymocytes to the thymic medulla, probably through repulsion of Sema3E. Endothelial cell Plexin D1 binding to Sema4A can oppose VEGF and suppresses tumor angiogenesis, and expression of Sema3E correlates inversely with tumor metastasis, indicating that Plexin D1 is anti-metastatic in the presence of its ligands.

Alternate Names

PLXND1

Entrez Gene IDs

23129 (Human)

Gene Symbol

PLXND1

UniProt

NP_055918. (Human)

Additional Plexin D1 Products

Product Documents for Plexin D1 Antibody

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Product Specific Notices for Plexin D1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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