PLGF Antibody (37203.111)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-10966
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 37203.111
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PLGF Antibody (37203.111)
Immunogen
Purified recombinant human placenta growth factor expressed in E. coli.
Localization
The three isoforms are secreted but PlGF2 appears to remain cell attached unless released by heparin.
Specificity
In immunoblotting, the shows no cross-reactivity with recombinanthuman VEGF, PDGFAA, PDGFAB, and PDGFBB.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for PLGF Antibody (37203.111)
Application
Recommended Usage
ELISA
2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: For capture ELISAs, this product can be used as the capture antibody. Use at a working concentration in combination with a biotinylated PlGF polyclonal detection antibody at 100 ng/ml (100 ul/well). WB: The detection limit for recombinant human placental growth factor is approximately 20 ng/lane and 300 ng/lane under non-reducing and reducing conditions, respectively. Homodimeric recombinant PlGF migrates as an approximately 29 kDa protein.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using NB120-10966 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 um filtered solution in PBS and carbohydrates
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PlGF
Long Name
Placenta Growth Factor
Alternate Names
PGF, PGFL
Entrez Gene IDs
5228 (Human)
Gene Symbol
PGF
UniProt
Additional PlGF Products
Product Documents for PLGF Antibody (37203.111)
Product Specific Notices for PLGF Antibody (37203.111)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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