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Key Product Details

Species Reactivity

Xenopus

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AZ44

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Baculo-expressed PLK1 from Xenopus laevis

Epitope

This antibody is thought to bind to a phosphorylated epitope. However, this has not yet been proved.

Localization

Nuclear

Specificity

Plx is activated by phosphorylation. AZ44 only detects the mitotic form of Plx (Xenopus laevis) which may be due to the recognition of a phosphorylated epitope. Recommended concentration: 1 ug/ml

Marker

Mitosis Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for PLK1 Antibody (AZ44) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): Xenopus egg extract.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLK1

Polo-like kinase (PLK) plays an important role in cell cycle regulation. It is required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a polo-like kinase kinase (PLKK) that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Entrez Gene IDs

5347 (Human); 18817 (Mouse)

Gene Symbol

PLK1

Additional PLK1 Products

Product Documents for PLK1 Antibody (AZ44) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PLK1 Antibody (AZ44) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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