Key Product Details

Species Reactivity

Xenopus

Applications

ELISA, Western Blot

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AZ44

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PLK1 Antibodies »

Product Specifications

Immunogen

Baculo-expressed PLK1 from Xenopus laevis

Epitope

This antibody is thought to bind to a phosphorylated epitope. However, this has not yet been proved.

Localization

Nuclear

Specificity

Plx is activated by phosphorylation. AZ44 only detects the mitotic form of Plx (Xenopus laevis) which may be due to the recognition of a phosphorylated epitope. Recommended concentration: 1 ug/ml

Marker

Mitosis Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for PLK1 Antibody (AZ44) [mFluor Violet 450 SE]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: PLK1

Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Gene Symbol

PLK1

Additional PLK1 Products

Product Documents for PLK1 Antibody (AZ44) [mFluor Violet 450 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PLK1 Antibody (AZ44) [mFluor Violet 450 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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