PLK5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97306

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PLK5 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human PLK5 protein (between residues 200-250) [UniProt Q496M5]

Localization

Nucleus, nucleolus. Cytoplasm.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLK5 Antibody

Western Blot: PLK5 Antibody [NBP1-97306]

Western Blot: PLK5 Antibody [NBP1-97306]

Western Blot: PLK5 Antibody [NBP1-97306] - WB analysis of PLK5 in 1. human brain lysate and 2. mouse brain lysate. Molecular weight of PLK5 differs between human and mouse species.
Immunocytochemistry/ Immunofluorescence: PLK5 Antibody [NBP1-97306]

Immunocytochemistry/ Immunofluorescence: PLK5 Antibody [NBP1-97306]

Immunocytochemistry/Immunofluorescence: PLK5 Antibody [NBP1-97306] - PLK5 antibody was tested in HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: PLK5 Antibody [NBP1-97306]

Immunohistochemistry: PLK5 Antibody [NBP1-97306]

Immunohistochemistry: PLK5 Antibody [NBP1-97306] - IHC analysis of PLK5 in mouse salivary gland using DAB with hematoxylin counterstain.

Applications for PLK5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:40

Immunohistochemistry

1:40-1:200

Immunohistochemistry-Paraffin

1:40-1:200

Western Blot

1:1000
Application Notes
This PLK5 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~50 kDa in human brain lysate and ~66 kDa in mouse brain lysate. Molecular weight of PLK5 differs between human and mouse species. In ICC/IF, nuclear staining was observed in HepG2 cells. In IHC-P, nuclear and cytoplasmic staining was seen in mouse salivary gland tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine (pH 7.5) and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLK5

PLK5 (PLK5P, Polo-like kinase 5) is a polo box domain-only protein that is down-regulated in proliferating cells and accumulates in quiescent cells. PLK5 is significantly silenced in brain cancer and overexpression of PKL5 triggers apoptotic effects, both of which suggest that it may have a tumor suppressor function. PLK5 has also been theorized to be important to the proper formation of neurite processes.

Alternate Names

MGC118807, MGC118808, PLK5P, polo-like kinase 5, polo-like kinase 5, pseudogene, SgK384ps

Entrez Gene IDs

126520 (Human)

Gene Symbol

PLK5

UniProt

Q496M5 (Human), Q4FZD7 (Mouse)

Additional PLK5 Products

Product Documents for PLK5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PLK5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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