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PLOD3 Antibody (2B1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43020

Novus Biologicals, part of Bio-Techne
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NBP3-43020

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 - (Uniprot# O60568)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLOD3 Antibody (2B1)

PLOD3 Antibody (2B1)

Western Blot: PLOD3 Antibody (2B1) [NBP3-43020] -

Western Blot: PLOD3 Antibody (2B1) [NBP3-43020] - human placenta tissue were subjected to SDS PAGE followed by western blot with (PLOD3 antibody) at dilution of 1:300
PLOD3 Antibody (2B1)

Immunohistochemistry: PLOD3 Antibody (2B1) [NBP3-43020] -

Immunohistochemistry: PLOD3 Antibody (2B1) [NBP3-43020] - Immunohistochemistry of paraffin-embedded human pancreas cancer using (PLOD3 antibody) at dilution of 1:100
PLOD3 Antibody (2B1)

Immunoprecipitation: PLOD3 Antibody (2B1) [NBP3-43020] -

Immunoprecipitation: PLOD3 Antibody (2B1) [NBP3-43020] - IP Result of anti-PLOD3 (IP:FNab06554, 5ug; Detection:FNab06554 1:500) with HeLa cells lysate 1400ug.

Applications for PLOD3 Antibody (2B1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Immunohistochemistry

1:20 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:200 - 1:1000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PLOD3

PLOD3 is encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq]

Alternate Names

2-oxoglutarate 5-dioxygenase 3, EC 1.14.11.4, LH3Lysyl hydroxylase 3, lysine hydroxylase 3, procollagen-lysine, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3

Gene Symbol

PLOD3

Additional PLOD3 Products

Product Documents for PLOD3 Antibody (2B1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PLOD3 Antibody (2B1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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