PLVAP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83911

Novus Biologicals, part of Bio-Techne
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NBP1-83911
NBP1-83911-25ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Electron Microscopy, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Electron Microscopy, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PLVAP Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLVAP Antibody

Western Blot: PLVAP Antibody [NBP1-83911]

Western Blot: PLVAP Antibody [NBP1-83911]

Western Blot: PLVAP Antibody [NBP1-83911] - Cell lysate from Human Retinal Microvascular Endothelial Cells (HRMECs). PLVAP antibody at a dilution of (1:1000) and overnight incubation at 4C. Left = untreated cells and Right = 24hr VEGFA treatment. Green band = PLVAP and Red band = Actin. Ladder is Odyssey 928-40000 molecular weight marker. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: PLVAP Antibody [NBP1-83911]

Immunohistochemistry-Paraffin: PLVAP Antibody [NBP1-83911]

Immunohistochemistry-Paraffin: PLVAP Antibody [NBP1-83911] - Human kidney. Heat mediated antigen retrieval in citrate buffer (pH 6) at 95C for 20 minutes. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: PLVAP Antibody [NBP1-83911]

Immunohistochemistry-Paraffin: PLVAP Antibody [NBP1-83911]

Immunohistochemistry-Paraffin: PLVAP Antibody [NBP1-83911] - Staining of human duodenum shows strong membranous positivity in endothelial cells.
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Applications for PLVAP Antibody

Application
Recommended Usage

Electron Microscopy

Reported in scientific literature (PMID:34280928).

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

Validated by verified customer review.
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 3 reviews rated 4.3 using NBP1-83911 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLVAP

PLVAP (Plasmalemma vesicle associated protein) is a membrane associated protein of caveolae and is found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Normally in skeletal and cardiac muscle, PLVAP expression is limited to small arterioles and venules; however, under conditions of inflammation, it can be induced on previously non expressing vessels in cardiac muscle. In the central nervous system (CNS), the panendothelial cell antigen expression is developmentally regulated. During embryonic development, the antigen is found on brain vasculature up to day 16 of gestation, after which it disappears. The cessation of PLVAP expression in the CNS may be associated with the establishment of the blood brain barrier, which begins on day 16 of gestation. In the adult mouse, inflammation in the CNS can lead to re expression of the panendothelial cell antigen. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Long Name

Plasmalemma Vesicle Associated Protein

Alternate Names

FELS, gp68, PV-1, PV1

Gene Symbol

PLVAP

Additional PLVAP Products

Product Documents for PLVAP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PLVAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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