PLZF Antibody (3A7)
Novus Biologicals, part of Bio-Techne | Catalog # H00007704-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3A7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ZBTB16 (AAH29812, 381 a.a. ~ 480 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QRELFSKLGELAVGMKSESRTIGEQCSVCGVELPDNEAVEQHRKLHSGMKTYGCELCGKRFLDSLRLRMHLLAHSAGAKAFVCDQCGAQFSKEDALETHR
Localization
Nuclear
Specificity
ZBTB16 - zinc finger and BTB domain containing 16
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for PLZF Antibody (3A7)
![Western Blot: PLZF Antibody (3A7) [H00007704-M01]](https://resources.bio-techne.com/images/products/PLZF-Antibody-3A7-Western-Blot-H00007704-M01-img0004.jpg "Western Blot: PLZF Antibody (3A7) [H00007704-M01]")
Western Blot: PLZF Antibody (3A7) [H00007704-M01]
Western Blot: PLZF Antibody (3A7) [H00007704-M01] - Analysis of ZBTB16 expression in transfected 293T cell line by ZBTB16 monoclonal antibody (M01), clone 3A7.Lane 1: ZBTB16 transfected lysate (Predicted MW: 11.11 KDa).Lane 2: Non-transfected lysate.![Proximity Ligation Assay: PLZF Antibody (3A7) [H00007704-M01]](https://resources.bio-techne.com/images/products/PLZF-Antibody-3A7-Proximity-Ligation-Assay-H00007704-M01-img0002.jpg "Proximity Ligation Assay: PLZF Antibody (3A7) [H00007704-M01]")
Proximity Ligation Assay: PLZF Antibody (3A7) [H00007704-M01]
Proximity Ligation Assay: PLZF Antibody (3A7) [H00007704-M01] - Analysis of protein-protein interactions between TRAF2 and ZBTB16. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ZBTB16 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: PLZF Antibody (3A7) [H00007704-M01] - Detection limit for recombinant GST tagged ZBTB16 is approximately 1ng/ml as a capture antibody.
Applications for PLZF Antibody (3A7)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Proximity Ligation Assay
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PLZF
Long Name
Promyelocytic Leukemia Zinc Finger
Alternate Names
ZBTB16, ZNF145
Entrez Gene IDs
7704 (Human)
Gene Symbol
ZBTB16
OMIM
176797 (Human)
UniProt
Additional PLZF Products
Product Documents for PLZF Antibody (3A7)
Product Specific Notices for PLZF Antibody (3A7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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