PMEL17/SILV Antibody (OTI10E5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47974

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat, Canine, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI10E5

Concentration

1.2 mg/ml

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Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human SILV expression vector

Specificity

This antibody is specific for Homo sapiens silver homolog (mouse) (SILV).

Marker

Melanosome Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

70.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PMEL17/SILV Antibody (OTI10E5)

Western Blot: PMEL17/SILV Antibody (OTI10E5) [NBP1-47974]

Western Blot: PMEL17/SILV Antibody (OTI10E5) [NBP1-47974]

Western Blot: PMEL17/SILV Antibody (10E5) [NBP1-47974] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMEL17/SILV(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMEL17/SILV.
Immunocytochemistry/Immunofluorescence: PMEL17/SILV Antibody (10E5) [NBP1-47974] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PMEL17/SILV.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (OTI10E5) [NBP1-47974]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (OTI10E5) [NBP1-47974]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (10E5) [NBP1-47974] - Staining of paraffin-embedded prostate tissue using anti-PMEL17/SILV mouse monoclonal antibody.
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Applications for PMEL17/SILV Antibody (OTI10E5)

Application
Recommended Usage

Flow Cytometry

1:500

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Reviewed Applications

Read 1 review rated 5 using NBP1-47974 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PMEL

Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity

Long Name

Premelanosome Protein

Alternate Names

Gp100, ME20, ME20-M, ME20M, PMEL17, SILV

Entrez Gene IDs

6490 (Human)

Gene Symbol

PMEL

UniProt

P40967 (Human)

Additional PMEL Products

Product Documents for PMEL17/SILV Antibody (OTI10E5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PMEL17/SILV Antibody (OTI10E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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