PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73479

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI10E5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

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Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human SILV expression vector

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

70.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free

Western Blot: PMEL17/SILV Antibody (OTI10E5)Azide and BSA Free [NBP2-73479]

Western Blot: PMEL17/SILV Antibody (OTI10E5)Azide and BSA Free [NBP2-73479]

Western Blot: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMEL17/SILV(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMEL17/SILV
Immunocytochemistry/ Immunofluorescence: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479]

Immunocytochemistry/ Immunofluorescence: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479]

Immunocytochemistry/Immunofluorescence: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PMEL17/SILV.
Immunohistochemistry: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479]

Immunohistochemistry: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479]

Immunohistochemistry: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479] - Staining of paraffin-embedded Kidney tissue using anti-PMEL17/SILV mouse monoclonal antibody.
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Applications for PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:500

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PMEL

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Long Name

Premelanosome Protein

Alternate Names

Gp100, ME20, ME20-M, ME20M, PMEL17, SILV

Gene Symbol

PMEL

Additional PMEL Products

Product Documents for PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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