PMS1 Antibody (PCRP-PMS1-2E11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13739

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-PMS1-2E11

Concentration

0.2 mg/ml

View all PMS1 Antibodies »

Product Summary for PMS1 Antibody (PCRP-PMS1-2E11)

Immunogen

Recombinant full-length human PMS1 protein (Uniprot: P54277)

Localization

Nucleus, Nuclear bodies

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

105.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14142)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for PMS1 Antibody (PCRP-PMS1-2E11)

Immunocytochemistry/ Immunofluorescence: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739]

Immunocytochemistry/ Immunofluorescence: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739]

Immunocytochemistry/Immunofluorescence: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PMS1 Antibody (PCRP-PMS1-2E11) [CF488]. PMS1 localized to nucleoplasm and nuclear bodies. Microtubules stained with CF640R.
Flow Cytometry: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739]

Flow Cytometry: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739]

Flow Cytometry: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739] - Flow cytometric analysis of PFA-fixed HeLa cells. PMS1 antibody (PCRP-PMS1-2E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739]

Protein Array: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739]

Protein Array: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PMS1 Antibody (PCRP-PMS1-2E11).

Applications for PMS1 Antibody (PCRP-PMS1-2E11)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: concentration is assay dependent

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PMS1

PMS1 was identified by its homology to a yeast protein involved in DNA mismatch repair. A role for this protein in mismatch repair has not been proven. However, the protein forms heterodimers with MLH1, a DNA mismatch repair protein, and some cases of hereditary nonpolyposis colorectal cancer have been found to have mutations in the gene.

Alternate Names

DKFZp781M0253, FLJ98259, HNPCC3, hPMS1, human homolog of yeast mutL, mismatch repair gene PMSL1, PMS1 postmeiotic segregation increased 1 (S. cerevisiae), PMS1 protein homolog 1, PMSL1DNA mismatch repair protein PMS1, postmeiotic segregation increased (S. cerevisiae) 1, rhabdomyosarcoma antigen MU-RMS-40.10B, rhabdomyosarcoma antigen MU-RMS-40.10E

Gene Symbol

PMS1

UniProt

P54277 (Human)

Additional PMS1 Products

Product Documents for PMS1 Antibody (PCRP-PMS1-2E11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PMS1 Antibody (PCRP-PMS1-2E11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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