PMS2 Antibody (163C1251) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56554
![Western Blot: PMS2 Antibody (163C1251)BSA Free [NB100-56554]](https://resources.bio-techne.com/images/products/PMS2-Antibody-163C1251-Western-Blot-NB100-56554-img0003.jpg "Western Blot: PMS2 Antibody (163C1251)BSA Free [NB100-56554]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 163C1251
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 623-639 SSLAKRIKQLHHEAQQS of human PMS2; GenBank No. NP_000526.1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for PMS2 Antibody (163C1251) - BSA Free
Western Blot: PMS2 Antibody (163C1251)BSA Free [NB100-56554]
Western Blot: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of PMS2 using this antibody at 2 ug/ml against 10 ug of NIH 3T3 cell lysate.![Immunohistochemistry-Paraffin: PMS2 Antibody (163C1251) - BSA Free [NB100-56554]](https://resources.bio-techne.com/images/products/PMS2-Antibody-163C1251-Immunohistochemistry-Paraffin-NB100-56554-img0005.jpg "Immunohistochemistry-Paraffin: PMS2 Antibody (163C1251) - BSA Free [NB100-56554]")
Immunohistochemistry-Paraffin: PMS2 Antibody (163C1251) - BSA Free [NB100-56554]
Immunohistochemistry-Paraffin: PMS2 Antibody (163C1251) [NB100-56554] - Analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using PMS2 antibody at 5 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of PMS2 was observed. Staining was performed by Histowiz.Applications for PMS2 Antibody (163C1251) - BSA Free
Application
Recommended Usage
Flow Cytometry
1ug per 10^6 cells
Immunohistochemistry
2-5ug/ml
Immunohistochemistry-Paraffin
2-5ug/ml
Western Blot
1-2 ug/ml
Application Notes
An approx. 85 kDa band should be observed. This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PMS2
Alternate Names
DNA mismatch repair protein PMS2, EC 3.1, H_DJ0042M02.9, HNPCC4postmeiotic segregation increased (S. cerevisiae) 2, mismatch repair endonuclease PMS2, PMS1 protein homolog 2, PMS2 postmeiotic segregation increased 2 (S. cerevisiae), PMSL2PMS2CL
Gene Symbol
PMS2
UniProt
Additional PMS2 Products
Product Documents for PMS2 Antibody (163C1251) - BSA Free
Product Specific Notices for PMS2 Antibody (163C1251) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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