PMS2 Antibody (4E5F1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16441

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16441-100ul
NBP3-16441-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4E5F1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PMS2 Antibodies »

Product Summary for PMS2 Antibody (4E5F1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PMS2 (P54278). MERAESSSTEPAKAIKPIDRKSVHQICSGQVVLSLSTAVKELVENSLDAGATNIDLKLKDYGVDLIEVSDNGCGVEEENFEGLTLKHHTSKIQEFADLTQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PMS2 Antibody (4E5F1)

Western Blot: PMS2 Antibody (4E5F1) [NBP3-16441]

Western Blot: PMS2 Antibody (4E5F1) [NBP3-16441]

Western Blot: PMS2 Antibody (4E5F1) [NBP3-16441] - Western blot analysis of extracts of various cell lines, using PMS2 Rabbit mAb (NBP3-16441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: PMS2 Antibody (4E5F1) [NBP3-16441]

Immunohistochemistry-Paraffin: PMS2 Antibody (4E5F1) [NBP3-16441]

Immunohistochemistry-Paraffin: PMS2 Antibody (4E5F1) [NBP3-16441] - Immunohistochemistry of paraffin-embedded human tonsil using PMS2 Rabbit mAb (NBP3-16441) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: PMS2 Antibody (4E5F1) [NBP3-16441]

Immunohistochemistry-Paraffin: PMS2 Antibody (4E5F1) [NBP3-16441]

Immunohistochemistry-Paraffin: PMS2 Antibody (4E5F1) [NBP3-16441] - Immunohistochemistry of paraffin-embedded Colon adenocarcinomas with loss of MLH1 expression(left) and Colon adenocarcinomas with loss of PMS2 expression(right) using PMS2 Rabbit mAb (NBP3-16441) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for PMS2 Antibody (4E5F1)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PMS2

PMS2 is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene.

Alternate Names

DNA mismatch repair protein PMS2, EC 3.1, H_DJ0042M02.9, HNPCC4postmeiotic segregation increased (S. cerevisiae) 2, mismatch repair endonuclease PMS2, PMS1 protein homolog 2, PMS2 postmeiotic segregation increased 2 (S. cerevisiae), PMSL2PMS2CL

Gene Symbol

PMS2

Additional PMS2 Products

Product Documents for PMS2 Antibody (4E5F1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PMS2 Antibody (4E5F1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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