PMVK/phosphomevalonate kinase Antibody (014)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90297
Recombinant Monoclonal Antibody
![Western Blot: PMVK/phosphomevalonate kinase Antibody (014) [NBP2-90297]](https://resources.bio-techne.com/images/products/PMVK-phosphomevalonate-kinase-Antibody-014-Western-Blot-NBP2-90297-img0001.jpg "Western Blot: PMVK/phosphomevalonate kinase Antibody (014) [NBP2-90297]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 014
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human PMVK/phosphomevalonate kinase (Uniprot#: Q15126; Met1-Leu192).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for PMVK/phosphomevalonate kinase Antibody (014)
Western Blot: PMVK/phosphomevalonate kinase Antibody (014) [NBP2-90297]
Western Blot: PMVK/phosphomevalonate kinase Antibody (014) [NBP2-90297] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:24 kDa(We are unsure as to the identity of these extra bands.)![Immunoprecipitation: PMVK/phosphomevalonate kinase Antibody (014) [NBP2-90297]](https://resources.bio-techne.com/images/products/PMVK-phosphomevalonate-kinase-Antibody-014-Immunoprecipitation-NBP2-90297-img0002.jpg "Immunoprecipitation: PMVK/phosphomevalonate kinase Antibody (014) [NBP2-90297]")
Immunoprecipitation: PMVK/phosphomevalonate kinase Antibody (014) [NBP2-90297]
Immunoprecipitation: PMVK/phosphomevalonate kinase Antibody (014) [NBP2-90297] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell Lysate0.5 uL anti-PMVK rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PMVK rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size: 21 kDaApplications for PMVK/phosphomevalonate kinase Antibody (014)
Application
Recommended Usage
ELISA
1:25000-1:50000
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PMVK/phosphomevalonate kinase
Alternate Names
hPMK, HUMPMKI, phosphomevalonate kinase, PMKA, PMKase, PMKEC 2.7.4.2, PMKI
Gene Symbol
PMVK
Additional PMVK/phosphomevalonate kinase Products
Product Documents for PMVK/phosphomevalonate kinase Antibody (014)
Product Specific Notices for PMVK/phosphomevalonate kinase Antibody (014)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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